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Each time one of the microscope objectives is changed, a corresponding adjustment must be performed on the substage condenser aperture diaphragm to adjust the numerical aperture of the illuminating cone of light to match that of the new objective.Ĭondenser Aperture Diaphragm Operation Explore how the substage condenser aperture controls illumination entering the objective. It is critical that the condenser light cone be properly adjusted to optimize the intensity and angle of light entering the objective front lens. More advanced optical microscopes are equipped with a substage condenser that gathers light from the microscope light source and concentrates it into an organized cone of light, which illuminates the specimen with uniform intensity over the entire objective rear focal plane. Light emitted from the QX3 stage diffusion screen travels upward in the direction of the objective front lens, but it is also reflected, scattered, and diffracted by the screen into a large number of point light sources, which hampers the ability to achieve illumination of consistent numerical aperture. The most serious drawback is a lack of organization of the illuminating light rays as they pass through the specimen. There are several problems associated with using this type of illumination for transmitted optical microscopy. Light emitted from this lamp is mixed within the confines of the mixing chamber and is then directed through a frosted plastic diffusion screen to illuminate a specimen placed on the microscope stage. In transmitted light mode, a small tungsten lamp positioned within a mixing chamber beneath the stage provides the source of illumination. The Intel Play QX3 Computer Microscope is capable of imaging specimens using either transmitted or reflected brightfield illumination. Molecular Expressions: Science, Optics and You - Intel Play QX3 Computer Microscope - High-Performance CondensersĪdvanced Condenser Systems High-Performance Condensers